Regulatory

Part:BBa_K145150:Design

Designed by: Jonas Demeulemeester   Group: iGEM08_KULeuven   (2008-08-06)

Hybrid promoter: HSL-LuxR activated, P22 C2 repressed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-35 and -10 boxes have been modified to lower the basal transcription rate. This part was constructing with an end-filling PCR reaction (with Klenow polymerase) of the following primers:

primer 1: CATCATGAAT TCGCGGCCGC TTCTAGACCT GTAGGATCGT ACAGGTTTAC TAAAGATTCC TTTAG
primer 2: TACTAGTAGC GGCCGCTGCA GGAAATTAAA GAACACTTAA ATTATAAACT AAAGGAATCT TTAG

Source

Synthetic

References