Regulatory
Part:BBa_K145150:Design
Designed by: Jonas Demeulemeester Group: iGEM08_KULeuven (2008-08-06)
Hybrid promoter: HSL-LuxR activated, P22 C2 repressed
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
-35 and -10 boxes have been modified to lower the basal transcription rate. This part was constructing with an end-filling PCR reaction (with Klenow polymerase) of the following primers:
primer 1: CATCATGAAT TCGCGGCCGC TTCTAGACCT GTAGGATCGT ACAGGTTTAC TAAAGATTCC TTTAG primer 2: TACTAGTAGC GGCCGCTGCA GGAAATTAAA GAACACTTAA ATTATAAACT AAAGGAATCT TTAG
Source
Synthetic